Retinal Molecular Changes Are Associated with Neuroinflammation and Loss of RGCs in an Experimental Model of Glaucoma

Signaling mediated by cytokines and chemokines is involved in glaucoma-associated neuroinflammation the damage of retinal ganglion cells (RGCs). Using multiplexed immunoassay immunohistochemical techniques a glaucoma mouse model at different time points after ocular hypertension (OHT), we analyzed (i) expression pro-inflammatory cytokines, anti-inflammatory BDNF, VEGF, fractalkine; (ii) number Brn3a+ RGCs. In OHT eyes, there was an upregulation IFN-? days 3, 5, 15; IL-4 1, 7 IL-10 3 5 (coinciding with downregulation IL1-? 7); (iii) IL-6 5; (iv) fractalkine VEGF day 1; (v) BDNF 7, 15. contralateral were IL-1? 1 coinciding IL4 7; 15 days; IL-17 reduction RGCs changes cytokine levels both eyes induction, confirming immune system involvement glaucomatous neurodegeneration.